The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis

Great Britain The Nucleotide Sequences Recognized by Endonucleases Aval and A vail from Anabaena variabilis

This sequence is cut as shown by the arrows to give single-stranded 5'-tetranucleotide protrusions (cohesive ends). Endonucleases SmaI, XhoI and XmaI recognize different symmetrical subsets of this sequence and provide independent evidence for the occurrence of these subsets at particular endonuclease-Aval cleavage sites in the bacteriophage-A genome. Further evidence for this structure came fr...

Bioenergetics Studies of the Cyanobacterium Anabaena variabilis

A cell-free system exhibiting both photophosphorylation (P /2e = 1) and oxidative phosphoryla­ tion (P/O up to 0 .8 ) is described for the cyanobacterium Anabaena variabilis. NADH and N A D PH were found to be equally effective as electron donors for oxidative phosphorylation. Low concentrations of U H D B T , an inhibitor of the cytochrome blc complex of mitochondria and chloroplasts. were fou...

Components of the proton electrochemical potential gradient in Anabaena variabilis.

RNA processing of nitrogenase transcripts in the cyanobacterium Anabaena variabilis.

Little is known about the regulation of nitrogenase genes in cyanobacteria. Transcription of the nifH1 and vnfH genes, encoding dinitrogenase reductases for the heterocyst-specific Mo-nitrogenase and the alternative V-nitrogenase, respectively, was studied by using a lacZ reporter. Despite evidence for a transcription start site just upstream of nifH1 and vnfH, promoter fragments that included ...

Potassium ion transport and electrogenesis in Anabaena variabilis.